After the run, look for . This report includes critical metrics such as:
STAR --runThreadN [threads] \ --genomeDir /path/to/index/ \ --readFilesIn read1.fastq read2.fastq \ --outFileNamePrefix SampleName_ Use code with caution. 4. Interpretation of the Report Download STAR bin
To download and use the (Spliced Transcripts Alignment to a Reference) binaries to generate a report, follow the steps below. This report is commonly used in bioinformatics to align RNA-seq reads to a reference genome. 1. Download STAR Binaries After the run, look for
The most direct way to obtain STAR is via its official GitHub repository. After the run
Before you can align reads and generate a report, you must create a genome index from your reference FASTA and GTF files.